Fig. 3

The fecal resistome composition of pigs. A Abundance of antimicrobial resistance genes (ARGs, i.e., number of reads aligned to ARGs within the MEGARes v2.0 database) by antimicrobial class over time. The ARGs counts were normalized using the CSS method. B Non-metric multidimensional scaling (NMDS) based on normalized count of ARG by AMR mechanism and biplot of AMR mechanism (annotated text represented AMR mechanism) showing overall changes in resistome composition with age. Polygons were applied to age groupings (Days 2–22, Days 26–77 and Days 93–179 of age. C Number of unique ARGs identified at each age-point. D, E Number of unique ARGs conferring resistance to drugs only, and ARGs conferring resistance to other categories (metal, multi-compounds, and biocides), respectively.). F Number of ARGs common within each age-group