Fig. 4

Observed ASV against disease status (a), sex (b), US state (c), farm (d), age (e), and breed purpose (f) evaluated with non-parametric Kruskal-Wallis and Mann-Whitney U tests. No significant differences in alpha-diversity with regard to disease status were observed with Obs ASV (p > 0.05). Significant differences in alpha-diversity were detected with regard to geographic location (both by state and farm, p < 0.0001), age (p < 0.0001), sex (p = 0.0379), and breed (p < 0.0001) for Obs ASV