Fig. 6

Relative expression of inflammatory cytokines presenting genes of methionine supplemented poultry primary intestinal epithelial cells. The gene expression levels related to pro- and anti-inflammatory cytokines were quantified using qRT-PCR and ∆C(t) values were normalized to internal control GAPDH. All values are expressed as mean ± SD; significant differences were determined using one-way ANOVA followed by Student’s t-test at *P < 0.05, **P < 0.005, ***P < 0.001, ****P < 0.0001 compared to blank