Fig. 4

GF mice colonized with EW microbiota demonstrate depression-like behavior and hyperactivity during the juvenile period. (A) Schedule for experiment 2. GF dam mice were orally administered EW or NW mice with fecal-derived microbiota or sterilized saline when the pups were PD10 (GF-NW, n = 23; GF-EW, n = 22; GF, n = 6). (B) Immobility time in the tail suspension test. Distance moved (C) and number of marbles buried (D) in the marble-burying test. The same letters in the line graph indicate a lack of significant differences between the groups. Data were represented as mean ± s.e.m. Comparison of performance in behavioral experiments at 4 and 8 wk of age; #p < 0.05, ##p < 0.01, ###p < 0.001