Fig. 5

Differential abundance of enzymes involved in A methane and B sulfur metabolism in the CON and PEO groups. Enzymes involved in these metabolic modules are shown in yellow. The blue text represents enzymes enriched in the PEO group, while the red text indicates enzymes enriched or tending to be enriched in the CON group. Inside the navy rectangles are the rumen microbiota that play an important role in the pathway. *P < 0.1, **P < 0.05, ***P < 0.01. Metabolic modules include: M00567: methanogenesis, carbon dioxide to methane, M00563: methanogenesis, methylamine/dimethylamine/trimethylamine to methane, M00357: methanogenesis, acetate to methane, M00356: methanogenesis, methanol to methane, M00596: dissimilatory sulfate reduction, sulfate to hydrogen sulfide (H2S)