Fig. 2

Microbial community alpha diversity and composition in giant grouper eggs (before and after ozone wash) and larvae. Box and whisker plots of alpha diversity metrics including (A) number of observed species, (B) Shannon diversity, and (C) Faith’s phylogenetic diversity calculated from rarefying samples to a depth of 2930 sequences 100 times. The thick centre within each box represents median values, and the upper and lower boundaries of the boxes represent the upper and lower quartiles, respectively. Whiskers extend to 1.5x interquartile range. (D) Principal component analysis (PCA) visualization of larvae-associated community composition over nine days. The PCA was performed on centered log ratio-transformed 16S counts as Euclidean distances. Each ring represents one replicate coloured by day of sampling. Coloured ellipses represent the 95% confidence limits of the group (sampling day) centroids