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Fig. 4 | Animal Microbiome

Fig. 4

From: The modulation of intestinal commensal bacteria possibly contributes to the growth and immunity promotion in Epinephelus akaara after feeding the antimicrobial peptide Scy-hepc

Fig. 4

Characterization of Scy-hepc absorption. a, representative microscopic images of the Scy-hepc absorption in different gut parts. Absorption—positive cells (red), nuclei are counterstained with DAPI (blue) (n = 6, independent animals). Scale bars: 100 μm. Light Gray arrow denote mucosa, olive arrow denote submucosa, lime green arrow denote muscularis and red arrow denote cavity. Samples were examined through confocal laser scanning microscopy. b, representative microscopic images of the Scy-hepc absorption in stomach. Light gray arrow denotes Gastric gland, olive arrow denotes stomach depression, lime green arrow denotes muscularis and dark orange denote blood vessel (n = 6, independent animals). c, Western blot showing the absorption signal of Scy-hepc in different tissues after gavage (St: stomach. Fg: foregut. Mg: midgut. Hg: hindgut. Lv: LIVER. Mu: muscle. Hk: head kidney. Bk: posterior kidney. Se: serum. Positive: positive control, n = 6, independent animals). d, Scy-hepc can be detected in serum through hybrid peptide by double antibody hybridization (n = 6, independent animals). Experiments replicated at least twice

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Animal Microbiome

ISSN: 2524-4671