Fig. 3
From: Fecal microbiome analysis uncovers hidden stress effects of low stocking density on rainbow trout
Quantitative polymerase chain reaction of the p4ha2 intron-10 retention marker compared to the upstream exon transcribed at steady state. The delta CT values obtained are inversely proportional to the intron-10 expression. Two-tailed t-tests revealed significant differences in the expression of the p4ha2 intron (p = 0.0044)