Fig. 8

Relative expression of immune, antioxidant defense and stress response genes. T-cell markers, a cluster of differentiation 3γδ, cd3γδ, b cluster of differentiation 8β, cd8β, other immune genes, c myeloid differentiation primary response gene 88, myd88, d major histocompatibility complex, class 1, mhc1, and e lysozyme C II, lysozyme C II, and goblet cell marker, f mucin 2, muc2, and antioxidant defense and stress response, g superoxide dismutase 1, sod1, h catalase, cat, and i heat shock protein 70, hsp70. Transcripts were quantified by qPCR and normalized using the geometric average of the selected reference genes. Values are presented as means ± S.E.M. (n = 6). Different letters among values indicate statistically significant differences (p < 0.05) in each group among the sampling points. Values sharing the same letters are not statistically significant. Any significant differences between the diet groups at a sampling point are reported in the text. Please refer to Fig. 1 legend for the description of the diet groups and sampling time points