Bacillus subtilis HGCC-1 improves growth performance and liver health via regulating gut microbiota in golden pompano

Table 3 Primer sequences for qRT-PCR analysis

Gene	Forward (5’ to 3’)	Reverse (5’ to 3’)	Size	Ref
tnf-α	GCTCCTCACCCACACCATCA	CCAAAGTAGACCTGCCCAGACT	/	[56]
il-1β	CGGACTCGAACGTGGTCACATTC	AATATGGAAGGCAACCGTGCTCAG	/	[56]
nf-kb	CGTGAGGTCAGCGAGCCAATG	ATGTGCCGTCTATCTTGTGGAATGG	158 bp	[56]
Il-8	CCGATCAACAGGGACTTCAA	GAGGACCGAGGGTTCAGACAG	/	[57]
ifn-γ	CCAGATGGATAACCTCGTCGT	AGCAGTGAACTCGCCTCGG	78 bp	[57]
il-10	AGTCAGTCTCCACCCCCATCTT	GCCCACTGGAGTTCAGATGCT	189 bp	[57]
tgf-β	GAGATACGGAAAAGAGTGGGG	TGACAAAGCGGGAAGCAAG	/	[58]
ppar-γ	TCAGGGTTTCACTATGGCGT	CTGGAAGCGACAGTATTGGC	150 bp	[59]
fasn	GAAGGAGAGGGGGTGGAGTC	GTGTGAAGGTGGAGGGTGTG	118 bp	[59]
acc1	GTTGTCAATCCCAGCCGATC	ATCCACAATGTAGGCCCCAA	/	[59]
hsl	TCATACCTCCACACCAACCC	GTCTCGCAGTTTCTTGGCAA	/	[59]
agpat3	CTTCCTGTTTTGGGCCACTC	GTCGCCATAACTTGAGCCTG	/	[59]
cpt1a	CATTGAGTCAGCTGCATTCTTC	CAGGGACTTGGCGTAACTATC	97 bp	[60]
ppar-α	GGAAATGCTTCGGATACTTG	AATCTCAGCGTGTCGTCTT	204 bp	[60]
cd36	CTCTTCATGGAAAGCAGGCC	CAGCTCCGAATACAACAGCC	/	[59]
fabp1	AGTCATTGTCTGGGGAGGG	GTCAAGGCGGTGGTTCA	198 bp	[61]
fabp2	CGGCTCCTGGAAAATTGATC	ATGGTTATCTTGAGGTTGTCGTG	111 bp	[62]
lpl	TTTGTCCTTCCTCGTCACCA	AAGACAGCATCCTCTCCACC	/	[59]
apob100	AAAAGCCACAAGACGAAAGCA	GAAGCAGCAAAAGGCAGAGC	128 bp	[60]
β-actin	TACGAGCTGCCTGACGGACA	GGCTGTGATCTCCTTCTGC	240 bp	[58]

Note: ‘/’ indicates the length of gene fragments amplified from the reference genome sequence according to Ref

ISSN: 2524-4671