Microbial profiling of black soldier fly larvae reared on substrates supplemented with different mineral sources originating from phosphorus recycling technologies

Reyer, Henry; Mielenz, Manfred; Daş, Gürbüz; Metges, Cornelia C.; Wimmers, Klaus

doi:10.1186/s42523-025-00380-5

Animal Microbiome

Table 2 Average relative abundance (%) of microbial genera in frass and intestinal samples from black soldier fly larvae reared on fly diet (FD) and FD supplemented with biochar (FD + BCH) or single superphosphate (FD + SSP)

From: Microbial profiling of black soldier fly larvae reared on substrates supplemented with different mineral sources originating from phosphorus recycling technologies

	Frass²				Larval intestine²				P-value
Genus¹	FD	FD + BCH	FD + SSP	SEM³	FD	FD + BCH	FD + SSP	SEM³	Sample origin
Achromobacter	1.57	2.26	2.01	0.63	0.06	0.11	0.24	0.08	0.001
Acinetobacter	11.28^a	8.81^a	3.39^b	2.03	0.39	0.86	0.44	0.35	< 0.001
Actinomyces	0.28	0.13	0.46	0.16	1.94	3.18	2.42	1.25	0.003
uncl. Actinomycetaceae	0.034^ab	0.001^b	0.119^a	0.04	0.98^a	0.10^b	2.21^a	0.70	< 0.001
uncl. Alcaligenaceae	0.53	1.20	1.79	0.46	0.10	0.23	0.15	0.07	0.002
Alcaligenes	1.55^b	5.07^a	2.36^ab	0.98	2.13	1.84	2.32	0.90	0.851
Ammoniphilus	0.10	0.06	0.09	0.02	0.65^a	0.35^a	0.06^b	0.13	0.003
Aquamicrobium	1.27	1.17	0.72	0.37	0.88	1.30	0.65	0.34	0.832
uncl. Bacillaceae	1.92	1.66	0.98	0.44	5.30	7.05	3.57	1.29	0.003
uncl. Bacillales	0.64	0.43	0.53	0.15	2.69^a	1.62^b	0.73^b	0.46	< 0.001
uncl. Bacteria	0.27	0.28	0.24	0.06	0.93	1.40	1.06	0.27	0.001
Brevundimonas	8.93^ab	13.74^a	2.62^b	3.29	1.93	3.89	1.74	0.87	0.006
Cellulosimicrobium	0.43	0.43	0.44	0.11	0.42	0.69	0.39	0.12	0.841
uncl. Enterobacteriaceae	2.15^b	0.91^b	6.62^a	0.62	0.94^b	1.90^ab	2.07^a	0.47	0.016
Enterococcus	0.31	0.41	0.62	0.13	0.53^b	1.02^b	2.10^a	0.44	0.053
Escherichia-Shigella	1.19	0.96	1.32	0.25	1.25^a	0.26^b	0.83^a	0.50	0.832
Flavobacterium	5.46	3.74	5.36	0.95	4.48	2.69	5.37	1.62	0.406
uncl. Lachnospiraceae	0.24	0.13	0.12	0.05	6.33	7.48	3.88	1.48	< 0.001
uncl. Lactobacillales	0.42	0.62	1.20	0.18	5.88	13.21	10.54	1.80	< 0.001
Methanobrevibacter	0.63	0.33	0.26	0.23	0.20^a	0.15^ab	0.05^b	0.04	0.363
uncl. Microbacteriaceae	0.98^a	0.27^b	1.01^a	0.16	0.67^a	0.46^b	0.90^a	0.16	0.230
Morganella	0.50	0.35	0.56	0.13	22.54^a	5.97^b	18.77^a	6.28	< 0.001
Ochrobactrum	6.82^b	3.69^b	13.23^a	0.97	3.99^b	2.92^b	7.06^a	1.17	0.023
Ornithinibacillus	0.50	0.08	0.51	0.20	0.57^a	0.06^b	0.10^ab	0.12	0.672
Paenibacillus	2.18	2.11	1.41	0.26	5.79	8.42	4.50	1.30	0.002
uncl. Planococcaceae	0.97^ab	0.77^a	0.3^b	0.14	4.89^a	5.13^a	0.34^b	0.68	< 0.001
Providencia	0.32	0.27	0.58	0.06	5.70	6.03	5.90	1.87	< 0.001
Pseudomonas	0.84^b	2.33^ab	8.03^a	1.77	0.002^b	0.031^b	0.149^a	0.041	< 0.001
Pseudoxanthomonas	5.21^ab	1.77^a	0.01^b	0.98	0.026^a	0.021^ab	0.002^b	0.010	< 0.001
Pusillimonas	5.55^a	3.76^a	0.19^b	1.30	1.85^a	1.03^a	0.12^b	0.46	0.152
Rhodococcus	0.30	0.22	0.25	0.06	0.32	0.55	0.24	0.08	0.786
uncl. Ruminococcaceae	0.08	0.06	0.05	0.04	0.60	0.89	0.18	0.19	0.003
Sphingobacterium	23.50	27.33	25.30	2.59	6.30	9.74	11.17	3.10	< 0.001
uncl. Sphingomonadaceae	0.89^ab	2.87^a	0.29^b	0.43	0.006^ab	0.063^a	0.003^b	0.030	< 0.001
Stenotrophomonas	1.27^b	3.13^a	7.85^a	1.03	0.04^c	0.32^b	0.72^a	0.12	< 0.001
Weissella	0.53	0.42	0.89	0.13	0.09^b	0.07^b	0.63^a	0.09	< 0.001

¹Genera with low abundance that failed the initial abundance filtering in the DESeq2 were excluded
²Six frass and six larval intestine samples were analysed for each substrate
³Pooled standard error of the mean for the relative abundance of microbial genera within frass and larvae
^abcDifferent superscripts indicate significant differences in microbial abundance between the substrates within frass and larvae, respectively (adjusted P-value < 0.05)

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