Fig. 5

Analysis of sample similarities and clustering in bacterial community studies. (a) Heatmap showing the RA of bacterial community composition, arranged according to samples similarity parameters. (b) Silhouette width plot for the different numbers of clusters. (c) and (d) Barplot representing the clusters assuming different number of k. Each bar represents the number of samples within each cluster, color-coded by sample type: L (Latxa), MRN (Manchega RN), MVL (Manchega VL), and R (Rasa). The asterisk (*) highlights the optimal K-value determined by the highest silhouette scores achieved in the analysis