Fig. 3

Microbial biomarkers. Linear discriminant effect size (LEfSe) analysis performed at the level of genus representing the significant bacterial biomarkers for each group (n = 5) and their abundance in normalized counts. The analysis was performed using all experimental groups (A) or only considering samples from the winter trial at 10 weeks post-exposure (wpe) (B) which were used for further analyses. Boxes represent the interquartile range (IQR) between the first and third quartiles and the vertical line inside the box defines the median. Whiskers represent the lowest and highest values within 1.5 times the IQR from the first and third quartiles. Samples with a relative abundance exceeding those values are represented as points outside the boxes. t1, t2, t3 stand for the different samplings performed at 2, 5 and 10 wpe, respectively. W and S stand for the different trials performed in winter and summer, respectively. C and R stand for control and parasite-exposed fish, respectively