Fig. 2

Species level comparison of shotgun metagenomic sequencing and deep culturing results. A hierarchy comparison of the bacterial species in this study was conducted to compare species identified by either culturing or shotgun metagenomic sequencing, with each terminal node representing a unique species. In A successfully cultured isolates which were identified to the species level using either MALDI-TOF MS or 16S rRNA gene sequencing are indicated using shade of pink, while grey is used for species only identified by metagenomics. In B species that were identified using shotgun metagenomic sequencing are indicated by a shade of blue, while nodes shown in grey indicate species for which isolates were obtained but which were not identified using shotgun metagenomics