Fig. 2

Effects of cooling on Alpha diversity indices, Beta diversity, and bacterial taxa relative abundances of the ruminal bacterial microbiota. A Ace index, B chao index, C Shannon index, D Simpson index, E principal coordinate analysis (PCoA) profile based on the unweighted Unifrac matrix, F PCoA profile based on the weighted Unifrac matrix, G Cladogram displaying significantly enriched bacterial taxa (LDA score > 3.0 and P < 0.05) from the phylum to the species level through the LEfSe analysis. Red: taxa abundant in CON; Blue: taxa abundant in MVWS, H LEfSe bar chat displaying the significantly differential taxa between treatments. The LDA scores represented the difference in relative abundance with exponent fold change of 10 across treatments