Fig. 3

Histological characteristics and bacterial distributions in the ANG of female bigfin reef squid. Dorsal (A) and ventral (B) views of the ANG in an adult female. Sagittal dissection of the ANG (C) and bacterial distribution (D). Tubular size and microbiota distribution in the dorsal (E) and ventral (F) regions. The arrows in the dorsal region (D) indicate the direction of the plan shown in the ventral regions (E and F). The distinct regions (yellow color) are characterized by morphology of epithelial cells and the staining color of the microbiota. Types of epithelial cells observed in secondary tubules include columnar epithelial cells (type 1; G and G’), cuboidal epithelial cells (type 2; H), squamous epithelial cells (type 3; 3 I), and transparent epithelial cells (type 4; J). The dash line denotes separate layers or regions based on their morphological differences. ANG, accessory nidamental gland; B, bacteria; Ci, cilia; CoEC, columnar epithelial cells; CuEC, cuboidal epithelial cells; Gi, gill; IEL, inner epithelial layer; IS, ink sac; NG, nidamental gland; Od, oviduct; OEL; outer epithelial layer; OG, oviductal gland; Ov, ovary; SEC, squamous epithelial cells; TEC, transparent epithelial cells