Fig. 4

Microbiomes and bacterial diversity of ANGs and their associated microbiota across different individuals. Microbiota distribution on the right side of the ANG collected from different individuals (A-F), with specific pigmented regions highlighted. Bacterial community composition (G) and mean relative abundance (H) of bacterial classes associated with whole ANGs (left side of ANG pair) and pigmented regions (right side of ANG pair) from different individuals. Bacterial classes with < 1% global relative abundances are presented as “Other”. Principal coordinate analysis (beta diversity) of bacterial classes (I) across whole ANGs and their associated pigmented regions, assessed by weighted UniFrac (ASV level) and Bray-Curtis distance (class level). The results of principal coordinate analysis of bacterial order and family are shown in Figure S2. Alpha diversity (J) indices of bacterial classes between whole ANGs and isolated pigmented regions. Observed indices of richness and Shannon’s and Simpson’s indices of diversity. O, orange; Sl, bigfin reef squid; W, white; Y, yellow