Microbial diversity and pigment synthesis in the accessory nidamental gland: species-specific and color-associated patterns in bigfin reef squid (Sepioteuthis lessoniana)

Table 2 Oligonucleotides of primers used in this study

Gene name	Oreintation	Sequence	Analysis
CO1	Sense	5’-GGTCAACAAATCATAAAGATATTGG-3’	Genotyping
CO1	Antisense	5’-TAAACTTCAGGGTGACCAAAAAATCA-3’	Genotyping
16S rRNA (V3-V4 region)	Sense	5’-CCTACGGGNGGCWGCAG-3’	Amplicon
16S rRNA (V3-V4 region)	Antisense	5’-GACTACHVGGGTATCTAATCC-3’	Amplicon
EF1A	Sense	5’-CCAGGTGACAATGTTGGTTTC-3’	qPCR
EF1A	Antisense	5’-GTCTCTTTGGGTGGGTTATTCT-3’	qPCR
ACSL	Sense	5’-CGTCGGAACAGACTTGAGATT-3’	qPCR
ACSL	Antisense	5’-ATGTGTGGATGGAAAGGATAGG-3’	qPCR
UGT	Sense	5’-CGTCTCTAGCTGTTGTTGATG-3’	qPCR
UGT	Antisense	5’-CCTGAAGGATGAAAGGAATCTG-3’	qPCR
ACL	Sense	5’-TTAGGCTGGTGTGTGTCATTAG-3’	qPCR
ACL	Antisense	5’-CGACTCTTGTCCGCATGTAA-3’	qPCR
NOS	Sense	5’-GTTCTGGATAGACCACAGGATTT-3’	qPCR
NOS	Antisense	5’-GTCAAGGAGGTTCGCAAGTTA-3’	qPCR
TGM1	Sense	5’-ACAGCAGCGTAACCATTGA-3’	qPCR
TGM1	Antisense	5’-GCAGGTCAGAACGAGACATATAA-3’	qPCR
PGRP2	Sense	5’-GGCACTATCTGCTGTTCAGAAT-3’	qPCR
PGRP2	Antisense	5’-GCACATCCCTGTGTCCAAATA-3’	qPCR

ISSN: 2524-4671