Fig. 2

Diversity analysis of the gut microbiota in olive flounder under varying temperature conditions. (A) Alpha diversity metrics: species richness (Chao1 index), diversity (Shannon index), and evenness (Pielou’s evenness) across temperature groups (18 °C, 22 °C, and 26 °C; n = 5). Statistical significance is indicated as *p ≤ 0.05 and **p ≤ 0.01; (B) Beta diversity analysis using the Bray-Curtis dissimilarity index, showing variation in gut bacterial community composition among the three temperature groups. Non-metric multidimensional scaling (NMDS) was applied, with stress value, R², and p-value provided for the analysis