Fig. 5

Predictive functional profiling of microbial communities in olive flounder across different temperature treatments using PICUSt2, focusing on KEGG pathways related to metabolism. The Y-axis represents the contributions to community-wide abundances of KEGG pathways, averaged across samples within each group (n = 5). (A) Carbohydrate metabolism, (B) Lipid metabolism, and (C) Amino acid metabolism pathways are depicted. Pathways are categorized into two levels: Level 2 pathways are shown at the top of each panel, represented by a gradient of black to gray colors to indicate varying degrees of pathway abundance. Level 3 pathways, which provide more detailed functional insights, are displayed at the bottom of each panel and are differentiated by purple, blue, and orange colors, respectively. Statistical significance of differences in pathway abundance between groups is indicated by asterisks: *p ≤ 0.05, **p ≤ 0.01