Fig. 6

Molecular networking and differential metabolite analysis in olive flounder across low and high temperature treatments. Molecular networking is visualized, where annotated metabolites are highlighted in pink, indicating their identification based on known databases. Metabolites with significant differences between temperature groups are marked in blue. The thickness of the connecting lines represents the cosine similarity score, indicating the structural similarity between metabolites. Accompanying the molecular network, a bar graph compares the intensity values of metabolites, representing their relative abundance or concentration. The low temperature group is depicted in blue, while the high temperature group is shown in green